separation of biojenic amines using dansyl cloride derivatization and mixed micellar liquid chromatography

separation of some biogenic amines via rp-hplc using mixed micellar mobile phase was investigated. the compounds were derivatized before hand by dansyl chloride as a chromophoric reagent. appropriate conditions for separation, were determined by studying factors such as temperature, type and percentage of organic modifier, concentration of surfactants (sds and brij-35) and the ph of the mobile phase. the number of theoretical plates (n), asymmetry factor (b/a) and selectivity factor (α) considered as criteria for finding the appropriate conditions for separation. appropriate condition, were: 40˚c, 40 mm sds, 0.5 mm brij-35 and 10% 1-propanol at ph=5.